Oxford Instruments HyperSense Dynamic Nuclear Polarizer
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|Contact Person||Malgorzata Marjanska (612) 625-4894|
Located near the 9.4-T, 31-cm horizontal bore magnet, the Oxford Instruments HyperSense enables a more than 10,000-fold enhancement of 13C NMR signals in solution [1, 2] using dynamic nuclear polarization (DNP) technique and a rapid dissolution procedure. This method not only facilitates the detection of the hyperpolarized substrate in vivo, but also the rapid imaging of cellular metabolism, with the downstream products being differentiated from the injected 13C-labeled substrate based on a change in chemical shift. Pyruvate is ideal for these studies because the hyperpolarized MR signal from the C1 carbon relaxes very slowly as a result of its long T1 and it is at the entry point to several important energy and biosynthetic pathways. In particular, it is converted to lactate in glycolysis, to alanine for protein synthesis and/or lipogenesis, and to acetyl-CoA and oxaloacetate to support the tricarboxylic acid cycle and biosynthesis of membrane lipids. Using hyperpolarized substrates, very high temporal resolution (on the order of seconds) data can be obtained and the observation of real-time, tissue specific metabolic changes is possible.
- Ardenkjaer-Larsen, J.H., et al., Increase in signal-to-noise ratio of > 10,000 times in liquid-state NMR. Proc Natl Acad Sci U. S. A., 2003. 100(18): p. 10158-10163.
- Golman, K., et al., Metabolic imaging by hyperpolarized 13C magnetic resonance imaging for in vivo tumor diagnosis. Cancer Res, 2006. 66(22): p. 10855-10860.